Quantitative measurement of lymphatic function in mice by noninvasive near-infrared imaging of a peripheral vein
Identifieur interne : 012A34 ( Main/Exploration ); précédent : 012A33; suivant : 012A35Quantitative measurement of lymphatic function in mice by noninvasive near-infrared imaging of a peripheral vein
Auteurs : Steven T. Proulx ; Qiaoli Ma ; Diana Andina ; Jean-Christophe Leroux ; Michael DetmarSource :
- JCI Insight [ 2379-3708 ] ; ????.
Abstract
Optical imaging methods have been developed to measure lymphatic function in skin; however, the lymphatic system of many organs is not accessible to this technology. Since lymphatic transport of macromolecules from any organ proceeds to the blood circulation, we aimed to develop a method that can measure lymphatic function by monitoring the fluorescence in a superficial vein of an interstitially injected tracer. We selected a 40-kDa PEGylated near-infrared dye conjugate, as it showed lymphatic system–specific uptake and extended circulation in blood. Lymphatic transport to blood from subcutaneous tissue required a transit time before signal enhancement was seen in blood followed by a steady rise in signal over time. Increased lymphatic transport was apparent in awake mice compared with those under continuous anesthesia. The methods were validated in K14-VEGFR-3-Fc and K14-VEGF-C transgenic mice with loss and gain of lymphatic function, respectively. Reduced lymphatic transport to blood was also found in aged mice. The technique was also able to measure lymphatic transport from the peritoneal cavity, a location not suitable for optical imaging. The method is a promising, simple approach for assessment of lymphatic function and for monitoring of therapeutic regimens in mouse models of disease and may have potential for clinical translation.
Url:
DOI: 10.1172/jci.insight.90861
PubMed: 28097238
PubMed Central: 5214224
Affiliations:
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Proulx</name></author><author><name sortKey="Ma, Qiaoli" sort="Ma, Qiaoli" uniqKey="Ma Q" first="Qiaoli" last="Ma">Qiaoli Ma</name></author><author><name sortKey="Andina, Diana" sort="Andina, Diana" uniqKey="Andina D" first="Diana" last="Andina">Diana Andina</name></author><author><name sortKey="Leroux, Jean Christophe" sort="Leroux, Jean Christophe" uniqKey="Leroux J" first="Jean-Christophe" last="Leroux">Jean-Christophe Leroux</name></author><author><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name></author></analytic><series><title level="j">JCI Insight</title><idno type="eISSN">2379-3708</idno><imprint><date when="????">????</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Optical imaging methods have been developed to measure lymphatic function in skin; however, the lymphatic system of many organs is not accessible to this technology. Since lymphatic transport of macromolecules from any organ proceeds to the blood circulation, we aimed to develop a method that can measure lymphatic function by monitoring the fluorescence in a superficial vein of an interstitially injected tracer. We selected a 40-kDa PEGylated near-infrared dye conjugate, as it showed lymphatic system–specific uptake and extended circulation in blood. Lymphatic transport to blood from subcutaneous tissue required a transit time before signal enhancement was seen in blood followed by a steady rise in signal over time. Increased lymphatic transport was apparent in awake mice compared with those under continuous anesthesia. The methods were validated in K14-VEGFR-3-Fc and K14-VEGF-C transgenic mice with loss and gain of lymphatic function, respectively. Reduced lymphatic transport to blood was also found in aged mice. The technique was also able to measure lymphatic transport from the peritoneal cavity, a location not suitable for optical imaging. The method is a promising, simple approach for assessment of lymphatic function and for monitoring of therapeutic regimens in mouse models of disease and may have potential for clinical translation.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Andina, Diana" sort="Andina, Diana" uniqKey="Andina D" first="Diana" last="Andina">Diana Andina</name><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name><name sortKey="Leroux, Jean Christophe" sort="Leroux, Jean Christophe" uniqKey="Leroux J" first="Jean-Christophe" last="Leroux">Jean-Christophe Leroux</name><name sortKey="Ma, Qiaoli" sort="Ma, Qiaoli" uniqKey="Ma Q" first="Qiaoli" last="Ma">Qiaoli Ma</name><name sortKey="Proulx, Steven T" sort="Proulx, Steven T" uniqKey="Proulx S" first="Steven T." last="Proulx">Steven T. Proulx</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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